Introduction of an improved direct agglutination test for the detection of Leishmania infantum infection in Southern France
Identifieur interne : 001509 ( Main/Exploration ); précédent : 001508; suivant : 001510Introduction of an improved direct agglutination test for the detection of Leishmania infantum infection in Southern France
Auteurs : P. M. De Korte [Pays-Bas] ; A. E. Harith [Pays-Bas] ; J. Dereure [France] ; E. Huigen [Pays-Bas] ; V. Faucherre [France] ; H. J. Van Der Kaay [Pays-Bas]Source :
- Parasitology Research [ 0932-0113 ] ; 1990-06-01.
Abstract
Abstract: An improved direct agglutination test (DAT) is assessed for the detection ofLeishmania infantum antibodies in man and in the canine reservoir in Southern France. The test performance in 32 human visceral leishmaniasis patients was in aggreement with the parasitological diagnosis and the immunofluorescence (IFAT) results. In six patients diagnosed earlier as kala-azar cases, both DAT and IFAT revealed negative results. The specificity of the DAT was reproduced in this study by the absence of cross-reactions with sera from other patients. In the dog reservoir, the DAT results were compared with those obtained by IFAT, the enzyme-linked immunosorbent assay (ELISA), cross-over electrophoresis and the latex agglutination test. All 34 dogs with parasitologically positive lymph node aspirates also had positive DAT titres (≤1∶640); the sensitivity in the other four tests was relatively low. Positive DAT and IFAT results were found in 16 unconfirmed cases of canine leshmaniasis, most probably due to prepatentL. infantum infections.
Url:
DOI: 10.1007/BF00931059
Affiliations:
- France, Pays-Bas
- Hollande-Méridionale, Hollande-Septentrionale, Languedoc-Roussillon, Occitanie (région administrative)
- Amsterdam, Leyde, Montpellier
- Université d'Amsterdam
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The test performance in 32 human visceral leishmaniasis patients was in aggreement with the parasitological diagnosis and the immunofluorescence (IFAT) results. In six patients diagnosed earlier as kala-azar cases, both DAT and IFAT revealed negative results. The specificity of the DAT was reproduced in this study by the absence of cross-reactions with sera from other patients. In the dog reservoir, the DAT results were compared with those obtained by IFAT, the enzyme-linked immunosorbent assay (ELISA), cross-over electrophoresis and the latex agglutination test. All 34 dogs with parasitologically positive lymph node aspirates also had positive DAT titres (≤1∶640); the sensitivity in the other four tests was relatively low. Positive DAT and IFAT results were found in 16 unconfirmed cases of canine leshmaniasis, most probably due to prepatentL. infantum infections.</div></front></TEI><affiliations><list><country><li>France</li><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li><li>Hollande-Septentrionale</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Amsterdam</li><li>Leyde</li><li>Montpellier</li></settlement><orgName><li>Université d'Amsterdam</li></orgName></list><tree><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="De Korte, P M" sort="De Korte, P M" uniqKey="De Korte P" first="P. M." last="De Korte">P. M. De Korte</name></region><name sortKey="Harith, A E" sort="Harith, A E" uniqKey="Harith A" first="A. E." last="Harith">A. E. Harith</name><name sortKey="Huigen, E" sort="Huigen, E" uniqKey="Huigen E" first="E." last="Huigen">E. Huigen</name><name sortKey="Van Der Kaay, H J" sort="Van Der Kaay, H J" uniqKey="Van Der Kaay H" first="H. J." last="Van Der Kaay">H. J. Van Der Kaay</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Dereure, J" sort="Dereure, J" uniqKey="Dereure J" first="J." last="Dereure">J. Dereure</name></region><name sortKey="Faucherre, V" sort="Faucherre, V" uniqKey="Faucherre V" first="V." last="Faucherre">V. Faucherre</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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